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KMID : 0900920020260010079
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Korean journal of Animal Reproduction
2002 Volume.26 No. 1 p.79 ~ p.85
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Effects of Cycloheximide on Development of In Vitro Matured Porcine Oocytes Activated following Eelectric Pulse
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Song Sang-Hyun
Jung Ki-Hwa
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Abstract
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We investigated the optimal concentration and exposure time of cycloheximide(CHX) on development of activated porcine oocytes following electrical pulse(EP). After 42~44 h maturation, oocytes were treated with 0.1% hyaluronidase, and denuded cumulus cells by pipetting. Oocytes were stimulated by electric pulse (1.2 kV/cm, 30 musec, 1 pulse) or incubated for 3, 5 and 7 h in cycloheximide (1, 5 and 10 mutextrm{g}/mell, respectively) following electric pulse, and cultured for 8 days. Cleavage rate of oocytes activated with 10 mutextrm{g}/mell CHX following EP was significantly (P＜0.05) higher than those of 1 mutextrm{g}/mell (86.8% vs. 74.4%). The developmental competence of oocytes incubated to 5 mutextrm{g}/mell of CHX was significantly (P＜0.05) higher development to blastocysts (13.3%), compared with 10 mutextrm{g}/mell of CHX (5.6%). When the oocytes were activated with 5mutextrm{g}/mell CHX for 3, 5, and 7 h following EP, the cleavage rate of oocytes in 5 h group(86.6%) was significantly (P＜0.05) higher than that in 3 h group(73.2%). The developmental rate of oocytes to morula in 5 and 7 h groups(26.7% and 16.4%) were significantly (P＜0.05) high than that in 3 h group(14.5%). Matured oocytes were activated with electric pulse (EP) or electric pulse combined with cycloheximide (EP + CHX) and cultured for 8 days. The rate of cleavage and development to blastocyst (80.1% and 11.6%) of activated with EP group were similar to EP combined with CHX group. When activated with EP or EP combined with CHX, the mean cell number of blastocysts were less in the activated with EP (18.67pm5.53) than in the activated EP combined CHX (20.71pm6.16), but not significantly different. This results suggest that, when the porcine oocytes were activated with CHX following EP, the developmental rate of activated oocytes can be improved by treated with a concentration of 5 mutextrm{g}/mell CHX for 5 h exposure time.
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KEYWORD
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Porcine oocyte, Activation, Electric pulse, Cycloheximide
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